plvx lentiviral backbone Search Results


lentiviral backbone plv ef1a ires puro  (Addgene inc)
96
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Lentiviral Backbone Plv Ef1a Ires Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral backbone plv ef1a ires neo  (Addgene inc)
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Lentiviral Backbone Plv Ef1a Ires Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral backbone plv hsyn rfp  (Addgene inc)
93
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Lentiviral Backbone Plv Hsyn Rfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral expression backbone  (Addgene inc)
93
Addgene inc lentiviral expression backbone
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Lentiviral Expression Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+lentiviral+backbone/pLV-U6-gRNA-UbC-eGFP-P2A-Bsr+(Plasmid+%2383925)/bio_rxiv__2021__03__08__434470-142-27-30
Average 93 stars, based on 1 article reviews
lentiviral expression backbone - by Bioz Stars, 2026-10
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lentiviral backbone  (Addgene inc)
95
Addgene inc lentiviral backbone
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Lentiviral Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+lentiviral+backbone/pLV-mCherry+(Plasmid+%2336084)/pmc12597689-121-13-15
Average 95 stars, based on 1 article reviews
lentiviral backbone - by Bioz Stars, 2026-10
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third generation lentiviral backbone  (Addgene inc)
95
Addgene inc third generation lentiviral backbone
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Third Generation Lentiviral Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+lentiviral+backbone/pLV-dCas9-p300-P2A-PuroR+(Plasmid+%2383889)/bio_rxiv__2025__07__11__659973-457-15-18
Average 95 stars, based on 1 article reviews
third generation lentiviral backbone - by Bioz Stars, 2026-10
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lentiviral backbone plasmids plvshctgf, plvscram, plv_iii_sod2  (Capital Biosciences)
90
Capital Biosciences lentiviral backbone plasmids plvshctgf, plvscram, plv_iii_sod2
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Lentiviral Backbone Plasmids Plvshctgf, Plvscram, Plv Iii Sod2, supplied by Capital Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral backbone plasmids plvshctgf, plvscram, plv_iii_sod2 - by Bioz Stars, 2026-10
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plvx lentiviral backbone  (Addgene inc)
94
Addgene inc plvx lentiviral backbone
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Plvx Lentiviral Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+lentiviral+backbone/pLVX-M-puro+(Plasmid+%23125839)/pmc07679775-69-7-11
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plvx lentiviral backbone - by Bioz Stars, 2026-10
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shao cong sun rrid addgene 71458 plvx ires mcherry takara bio 631237 pusepr  (Addgene inc)
88
Addgene inc shao cong sun rrid addgene 71458 plvx ires mcherry takara bio 631237 pusepr
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Shao Cong Sun Rrid Addgene 71458 Plvx Ires Mcherry Takara Bio 631237 Pusepr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+lentiviral+backbone/pCLXSN(GFP)-HA-Slc1a5+(mouse)+(Plasmid+%2371458)/pm38165806-190-80-82
Average 88 stars, based on 1 article reviews
shao cong sun rrid addgene 71458 plvx ires mcherry takara bio 631237 pusepr - by Bioz Stars, 2026-10
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lentiviral expression vector backbone plvu ttr krab  (Addgene inc)
92
Addgene inc lentiviral expression vector backbone plvu ttr krab
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Lentiviral Expression Vector Backbone Plvu Ttr Krab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a pet28b 6x his ulp1403 621 steven markus n a plv egfp lentiviral backbone  (Addgene inc)
95
Addgene inc paper n a pet28b 6x his ulp1403 621 steven markus n a plv egfp lentiviral backbone
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Paper N A Pet28b 6x His Ulp1403 621 Steven Markus N A Plv Egfp Lentiviral Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+lentiviral+backbone/pLV-eGFP+(Plasmid+%2336083)/pm38971154-503-110-123
Average 95 stars, based on 1 article reviews
paper n a pet28b 6x his ulp1403 621 steven markus n a plv egfp lentiviral backbone - by Bioz Stars, 2026-10
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plv ef1a ires hygro lentiviral backbone  (Addgene inc)
94
Addgene inc plv ef1a ires hygro lentiviral backbone
(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for <t>lentiviral</t> delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.
Plv Ef1a Ires Hygro Lentiviral Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plvx+lentiviral+backbone/pLV-EF1a-IRES-Hygro+(Plasmid+%2385134)/bio_rxiv__2022__12__16__520816-170-19-22
Average 94 stars, based on 1 article reviews
plv ef1a ires hygro lentiviral backbone - by Bioz Stars, 2026-10
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Image Search Results


(A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for lentiviral delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.

Journal: bioRxiv

Article Title: Genome-wide annotation of gene regulatory elements linked to cell fitness

doi: 10.1101/2021.03.08.434470

Figure Lengend Snippet: (A) Overall schematic of (i) discovery wgCERES screen, (ii) secondary validation screen of regulatory elements, (iii) cell-type specificity, and (iv) single-cell (scCERES) readout to connect cell fitness-associated regulatory elements to target genes. (B) A schematic of wgCERES approach. gRNAs are designed to all DHSs in the K562 cell line and synthesized as a pool for lentiviral delivery. K562 cells either constitutively expressing or not expressing dCas9 KRAB are treated with the lentiviral gRNA library at a low MOI and cultured for 14 population doublings. Genomic DNA is then harvested and the gRNA abundance is quantified by Illumina sequencing. (C) A schematic describing four levels of gRNA grouping analyses, including individual gRNA, sliding windows of 2 or 3 gRNAs, and averaging all gRNAs within a DHS. (D) Summary of DHS hits identified by significant changes to individual gRNAs or grouped gRNAs. (E) Volcano plot of significance of gRNA changes relative to log2(fold-change). (F) Distribution of significant gRNAs relative to transcriptional start sites of nearest genes. (G) Representative examples of significant distal DHS hits (blue boxes) that also have a significant DHS hit at a TSS of the nearest gene. ChromHMM tracks indicate promoters (red), putative enhancers (yellow), and polycomb repressed regions (gray). (H) UMAP dimensionality reduction plot showing different ChromHMM chromatin state informed classes of significant (FDR < 0.1) DHS hits. Histone modifications as well as several epigenetic modifying proteins were included as input for dimensionality reduction. (I) Relative abundances of significantly depleted or enriched gRNAs relative to ChromHMM classes of genome annotations.

Article Snippet: The lentiviral Grna expression plasmid was cloned by combining a U6-gRNA cassette containing the gRNA-(F+E)-combined scaffold sequence ( ) with an EGFP-P2A-PAC or mCherry-P2A-PAC cassette into a lentiviral expression backbone (Addgene #83925) using Gibson assembly.

Techniques: Biomarker Discovery, Synthesized, Expressing, Cell Culture, Illumina Sequencing